ABSTRACT
Objective@#To explore the influence of music training on the response inhibition ability of children with developmental dysplasia, and to provide a theoretical basis for improving the response inhibition ability of children with developmental dyslexia.@*Methods@#From September to October 2020, students from grades 3-6 in a primary school in Shenyang, Liaoning Province were selected. A total of 27 children with dyslexia were selected through literacy test and intelligence test, and 23 children with matched reading level were selected. The Go/No-go experimental paradigm was used to investigate the changes of response inhibition in children with developmental dyslexia before and after ALF music training, induding solfeggio, physical rhythm, music scene performance and chorus.@*Results@#The results before and after music training showed that the main effect in the test stage was significant among two groups[ F(1,48)=6.13, P<0.05, η-p 2=0.11], and The accuracy of post-test [(91.80±0.80)%] was significantly higher than that of pre-test [(89.10±0.90)%]; the accuracy of the children with developmental dyslexia in response to the symbolic stimulus No-go was significantly higher in the post-test [(81.81±10.97)%] than in the pre-test [(73.78±15.26)%]( t =-2.33, P = 0.03 ); the accuracy of reading matched children s response to Chinese characters stimulation No-go was significantly better in the post-test [(85.59±12.11)%] than in the pre-test [(78.33±12.98)%]( t = -2.20, P <0.05). In terms of response time, the post-test scores of developmental dyslexia children [(444.06±77.49)ms] were significantly better than those of pre-test children [(519.01±70.75)ms], and there was no significant difference between symbol stimulus and Chinese stimulus in developmental dyslexia children ( P>0.05). @*Conclusion@#Response inhibition is deficient in children with developmental dyslexia. Compared with symbols, the response inhibition ability of Chinese characters is impaired; Music training significantly improved the inhibitory ability of signs in children with developmental dyslexia.
ABSTRACT
Objective:To investigate the effects of miR-363-5p on the proliferation and apoptosis of nasopharyngeal carcinoma cells and the possible mechanism.Methods:miR-363-5p expression in human normal nasopharyngeal epithelial cells NP-69 and nasopharyngeal carcinoma cells 5-8F was detected using quantitative real-time polymerase chain reaction. Proliferation and apoptosis of 5-8F cells overexpresing miR-365-5p were determined. At the same time, Caspase3 and BRD4 protein expression in 5-8F cells were also detected.Results:miR-365-5p expression in 5-8F cells (0.71 ± 0.45) was significantly lower than that in NP-69 cells ( t = 2.68, P < 0.05). After overexpressing miR-363-5p, the proliferation of 5-8F cells was significantly decreased ( F = 22.68, P < 0.05). The apoptotic rate in the 5-8F cells was significantly higher than that in the control group [(24.45 ± 5.38)% vs. (18.23 ± 2.41)%, t = 4.13, P < 0.05]. Bax and Caspase3 protein levels in the 5-8F cells were (1.35 ± 0.24) and (1.44 ± 0.34) respectively, which were significantly higher than those in the NP-69 cells [(1.00 ± 0.08), (1.00 ± 0.23), t = 3.12, 5.12, P < 0.05]. BRD4 protein level in the 5-8F cells was significantly lower than that in the control group [(0.42 ± 0.24) vs. (1.00 ± 0.37), t = 2.98, P <0.05]. Conclusion:miR-365-5p can inhibit proliferation of nasopharyngeal carcinoma cells and promote their apoptosis. The negative regulatory effects of miR-363-5p on tumor cells are achieved possibly through inhibiting BRD4 protein expression.
ABSTRACT
Objective@#To examine the relationship between inhibition of ongoing responses deficit with attention deficit and behavior disorder among ADHD children.@*Methods@#Totally 43 children with attention deficit hyperac tivity disorder (ADHD) aged 6-14 years who met the DSM-V criteria for ADHD were recruited. And 38 health children were recruited as the control group. Vision Continuous Performance Task (CPT) and Matching Familiar Figures Test were used to assess attention deficit and behavior disorder feature of the ADHD children. Stop-signal paradigm was used to assess the inhibition of ongoing responses.@*Results@#In the stop-signal paradigm, the ADHD children’s error rate was significantly higher than typically developing children(t=4.34, P<0.01). The ADHD children error rate in the stopsignal task was positively correlated with the wrong number miss rate in CPT task(r=0.45, P<0.01), similar finding was observed in regression analysis,with inhibition of ongoing responses accounted for 20.1% of attention deficit(t=3.21,P<0.01). In ROC curve analysis, the cut-off sore was 15.50 in the task. The error rate in stop-signal task showed no association with miss rate in Matching Familiar Figures Test(r=0.09,P>0.05).@*Conclusion@#Inhibition of ongoing responses among ADHD children is associated with attention deficit, but not with existence of behavior disorder.
ABSTRACT
Objective To explore the correlation between circadian clock gene clock circadian regulator (CLOCK) and attention-deficit/hyperactivity disorder (ADHD) and the role of CLOCK and sleep problems on inhibition in male children with ADHD. Methods Two single nucleotide polymorphisms (SNPs) of CLOCK were genotyped in 854 male ADHD children and 320 male controls. Sleep problems were assessed using parent symptom questionnaire. In ADHD cases, the main effects and interaction of CLOCK SNPs and sleep problems on inhibition assessed by using Stroop Color and Word Test, were analyzed using the analysis of covariance (ANCOVA). Results No significant differences of allele and genotype frequencies were found for rs6832769 and rs11932595 in all case-control groups (P>0.05). In ADHD cas?es, the main effects of rs6832769 and rs11932595 genotypes and sleep problems on inhibition were not significant (P>0.05). However, the interaction of rs6832769 genotype and sleep problems was significant (F=6.71, P=0.01). When ac?companied with sleep problems, ADHD cases carrying the AA&AG genotype showed the longest time of word interfer? ence (F=6.63, P=0.01). Conclusions Inhibition of male ADHD children can be modulated by the interaction of CLOCK rs6832769 and sleep problems.
ABSTRACT
Objective To explore the reactive inhibition and electrophysiological changes under the GO/NOGO paradigm in patients with alcohol dependence (AD) in order to provide a theoretical basis for neuromechanism study of AD executive dysfunctions.Methods The GO/NOGO paradigm event related potentials (ERP) were tested in 51 AD patients and 29 healthy controls, this paradigm behavior and ERP-N1, N2 and P3 latency and amplitude were analyzed.ERPs and execution functions were followed at different time points of withdrawal(4, 9,13 weeks).Results The number of correct responses (29.18±3.03)lowered and error responses (5.16±3.77) heightened in AD patients, and differences had statistical significance compared with those in control group(32.35±2.58,3.19± 1.84, P＜0.05), and there was no group difference in response time.There was no statistical significance in group difference in N1 component under the GO/NOGO tasks and in P3 latency under GO.N2 ((3.48±2.21) μV, (4.49±3.00) pV) and P3 amplitude ((3.47±2.31) μV, (3.92±4.09) μV) decreased and N2 latency ((256.30±31.62) ms, (256.09±33.77)ms) prolonged in AD group under both GO/NOGO tasks and so did P3 latency ((355.33±25.96) ms) under the NOGO, and the differences had statistical significance.Group main effects in N2 and P3 component (P=0.000) and task main effects in N2 amplitude and P3 latency (P＜0.05) were significant,group and task interaction was significant only in P3 latency (P＜0.01).ERPs of AD patients had no notable changes at different time points of withdrawal, executive dysfunctions were the worst within the 1st week of withdrawal and gradually recovered to pre-withdrawal levels.Conclusion AD patients have lower reactive inhibition manifested mainly by weakened capacity to inhibit and adjust conflict monitor and pre-executive motor program, ERP and executive functions cannot be well improved during short-term withdrawal, and the GO/NOGO-ERP can completely reflect electrophysiological changes of reactive inhibition process of AD patients.